BIOL 102 Chapter Notes - Chapter 21: Dna Barcoding, Metagenomics, Chromosome
2 Nov 2018
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Divide up entire genome/transcriptome and figure out which part is of interest. Use known sequence or sequence similarity to design primers to amplify the region of interest. The nucleotides are added to the 3-prime end of the primer. If the dna polymerase adds a ddntp instead of dntp, the. You know what base was at what strand because they are colour coded. Cut the dna from many copies of a chromosome. Dna barcoding- identifying unknown species from short sequences. Metagenome- collection of dna obtained in a complex sample. Focusing on a target gene known to vary between species. Collect metagenome and determine every sequence of that gene in the sample. The number of copies of that gene in the sample indicates the abundance of the organism. Exon- sequences that get spliced together to make mature rna. Introns- sequences that separate exons on a chromosome. Promoter- the upstream region that regulate a gene.
