BIOCHEM 2B03 Chapter Notes - Chapter 6: Small Interfering Rna, Lentivirus, Islamic Republic News Agency
27 Nov 2015
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A: poly(a) binding protein (pabp) bind to the poly(a) tail, so mrna can now interact with pabp, pabp + eif4g loops mrna to prevent degradation, deadenylases shortens the poly(a) tail from 80 nucleotides 20 25 nucleotides = opens circular mrna, pan2 pan3, pabp dependent poly(a) nucleases inhibited by pab; no effect on 5" cap, ccr4 not stimulated by pab; effect on 5" cap unknown, parn, (poly(a)specific ribonuclease) inhibited by pab; stimulated by 5" cap structure. Ras probe: perform emsa assay, cerate radiolabelld ras probe specifically for. Ejc (exon junction complex) are added during/after splicing: can distinguish ptc from proper stop codons, ribosome encounters ptc and interacts with ejc downstream via direction interaction with upf1/2, upf1/2/3 are up frameshift proteins , form the core of the nmd machinery, upf1 has reversible phosphorylation properties, upf1 is phosphorylated activation of nmd, ribosome dissociates from ptc and upf1/2/3, upf1 is dephosphorylated by smg 6, smg5/7 and.
