#1 You decide to identify the CFTR mutation by analyzing the genomic DNA of your patients compared to healthy individuals. You specifically are looking to see whether a specific 3' gene truncation has occurred in the patients. You will determine this using hybridization techniques with samples from healthy and CF patients. Which of the following will allow you to accomplish this?
A) Using an RNA probe complementary to the region not removed by the truncation
B) Using an RNA probe complementary to the region removed by the truncation.
C) Using an DNA probe complementary to the region not removed by the truncation.
D) Using an DNA probe complementary to the region removed by the truncation
E) Using an antibody complementary to the region not removed by the truncation.
F) Using an antibody complementary to the region removed by the truncation.
#2To conduct the hybridization experiment, you are trying to decide between using a DNA or RNA probe. Which would be ideal to use and why?
A) As both are composed of nucleic acids, using either would result in identical results.
B) An RNA probe because RNA has uracil bases.
C) A DNA probe because it is more stable than RNA.
D) A DNA probe because RNA cannot bind to DNA.
#3Which of the following will lower the Tm of a given DNA strand?
A)Increasing the percentage of GC base pairs
B) Raising the pH of the solution from extremely acidic to neutral
C) Decreasing the buffer concentration from 50mM NaCl to 5mM NaCl.
#4 Imagine Hershey/Chase had used an RNA virus (genome composed of RNA) instead of a DNA virus in their experiment. Would radioactivity still have been found in the pellet?
A) No, because the RNA genome would not enter the bacteria upon infection
B) No, because while DNA and RNA nucleotides are similar, they are not identical
C) Yes, because DNA and RNA nucleotides are similar
D) Yes, because genome in any form (DNA, RNA, protein) would be labeled similarly.
#5 Griffith and Avery's transformation experiments allowed us to identify that DNA is our genetic information. Which of the following scenarios would result in bacterial cells that are capable of killing mice upon injection?
A) Heat killed non-virulent bacteria is added to a live virulent bacteria strain
B) Heat killed virulent bacteria is added to a heat killed non-virulent bacteria strain.
C) Heat killed mouse cells are added to a non-virulent bacteria.
#6 The human genome consists mostly of non-coding DNA. Which of the following are benefits of this?
A) Random DNA mutations generally won't affect RNA and protein function
B) It is faster to duplicate the genome when these are present.
C) The existence of introns can lead to multiple variations of proteins encoded by a single gene.
D) It is unlikely transposons would exist in the genome if there was too much protein coding DNA.
