BIOL 261 Chapter 10: Chap 10
27 Feb 2017
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10. 1 overview: isolating and amplifying specific dna fragments. Amplification = replication that allows for the isolation of quantities of dna to analyze. Donor dna (sample), in viva most often entire genome. Fragments of the donor = inserted into a specifically designed plasmid or bacterial virus that will carry + amplify the gene of interest vectors. First: donor dna molecules cut up by using enzymes restriction endonucleases . Cut along chromosome size dna molecules into hundred of thousands of as molecular scissors fragments of more manageable size. Second: each fragment is inserted into a cut vector to form recombinant dna molecules. Recombinant dna molecules transferred into bacterial cells + generally only 1 recom molecule is taken up by each cell. In each bacterial cell recombinant molecule = amplified along vetor during cell division. From single cell process results in a clone of identical cells each containing recombinant dna molecule.
