MICR-4010 Study Guide - Midterm Guide: Acridine Orange, Sybr Green I, Temperature Gradient Gel Electrophoresis
14 Oct 2018
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Class 12: briefly differentiate between culture-dependent and culture-independent methods to study microbes from the environment. Culture dependent: relies on whats being made in lab. Doesnt truly reflect the environment of the organism that is being grown. Culture independent: what nutrients/conditons the microbe has in its natural environment. How is that different than dilution to extinction or other methods to isolate cells prior to culturing: be able to name some examples of stains used in microbiology. Abiotic factors: lithosphere (rocks, sediment, soil) , hydrosphere (water, ocean) atmosphere (what we and microbes breath) Aquatic environments: abiotic factors involve proximity to surface, light/temperature, ph, salinity, nutrients. Biotic factors involve microbial metabolisms and microbial interactions. Terrestrial: abiotic factors that affect terrestrial environments are rock, presence of water in the case of water logged soil, different types of soil particles, sunlight, placement in the horizon.
