Question

The following data were collected for an unknown reactionmechanism for an enzyme catalyzing two substrates. The secondsubstrate concentrations were as follows: 2.00 x 10^-6 Mfor experiment 1, 4.00 x 10^-6 M for experiment 2, 6.00 x10^-6 M for experiment 3, and 8.00 x 10^-6 Mfor experiment 4. Determine whether the reaction is a sequential orping-pong mechanism, and determine all of the kinetic constants(K_M1, K_M2,1, v_max, etc.if applicable) associated with that type of raction mechanism.

	exp 1	exp 2	exp 3	exp 4
Concentration [S1] (M)	v2 (M/s)	v2 (M/s)	v2 (M/s)	v2 (M/s)
1 x 10-6	2.15 x 10-5	3.08 x 10-5	3.60 x 10-5	3.94 x 10-5
3 x 10-6	2.88 x 10-5	4.84 x 10-5	6.27 x 10-5	7.35 x 10-5
6 x 10-6	3.15 x 10-5	5.65 x 10-5	7.69 x 10-5	9.38 x 10-5
9 x 10-6	3.25 x 10-5	5.98 x 10-5	8.32 x 10-5	1.03 x 10-4

Note: Plotting in excel will be useful!