A 30.14 mg sample of protein was digested to convert all of thenitrogen present to ammonia.
After treatment the sample mixture was diluted to 100 mL. A 10.0mL portion of this mixture
was placed in a 50 mL flask and treated with excess phenol andhypochlorite and diluted to the
mark. The absorbance at 625 nm of this solution in a 1.00 cmcuvet was 0.605.
A 10 mL portion of standard NH4Cl containing 0.200 mg NH4Cl permL was added to a 50 mL
flask, treated with excess phenol and hypochlorite and dilutedto the mark as above. The
absorbance at 625 nm was 0.502 in a 1.00 cm cuvet.
A blank was prepared by adding phenol and hypochlorite reagentsto a 50 mL flask and diluting
to the mark. The absorbance was 0.114 in a 1.00 cm cuvet at 625nm.
Calculate the percentage by weight of nitrogen in the proteinsample.
A 30.14 mg sample of protein was digested to convert all of thenitrogen present to ammonia.
After treatment the sample mixture was diluted to 100 mL. A 10.0mL portion of this mixture
was placed in a 50 mL flask and treated with excess phenol andhypochlorite and diluted to the
mark. The absorbance at 625 nm of this solution in a 1.00 cmcuvet was 0.605.
A 10 mL portion of standard NH4Cl containing 0.200 mg NH4Cl permL was added to a 50 mL
flask, treated with excess phenol and hypochlorite and dilutedto the mark as above. The
absorbance at 625 nm was 0.502 in a 1.00 cm cuvet.
A blank was prepared by adding phenol and hypochlorite reagentsto a 50 mL flask and diluting
to the mark. The absorbance was 0.114 in a 1.00 cm cuvet at 625nm.
Calculate the percentage by weight of nitrogen in the proteinsample.
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