Glucose labeled with 14C in C-1 is completely converted to acetyl-CoA via glycolysis and the pyruvate dehydrogenase complex enters Krebs cycle. in which position (s) of the oxaloacetate 14C label be found?

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Related questions

a) Metabolicpathways have often been studied through the use of radioactivesubstrates.

Suppose glucose that was labeled with

14C at C1 (the aldehyde carbon) proceededthrough

glycolysis. Where would the label appear in theproduct, pyruvate? Draw the structure of

pyruvate and indicate the labeled carbon.

b) If Carbons 3 and 4 of glucose were labeledwith

14C and the glucosewas fermented to

ethanol, where would the labels appear? Explain.

Labeled pyruvate containing 14C ( 200 mCi/gm at ) C-3 ( the methyl carbon), 13 C( 90% enriched) at C-2 and 18 O ( 80%enriched) at C-2m was added to a pigeon breast muscle preparation, in order to investigate the citric acid cycle.

What will the labeling patern be ( both postion and extent of labeling ) in the isolated a- ketogluterate and in oxaloacetate ( Assume label enters onlin in the first turn ( ie pulse of labeled pyruvate is followed by a chase of unlabeled pyruvate.))