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13 Dec 2019

Solution Chemistry, please show, explain work!!â Calculate the amount (in mg) of Trizma Base (molar Mass 121.1) required to make 50 mL OF 50.0 micro Moles Tris buffer.

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17 Dec 2019

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Consider a 1.00 L buffer prepared by mixing 610 mg of Tris [tris(hydroxymethyl

aminomethane] (pKa = 8.07) in the free-base form (FW = 121.1 g/mol) and 4 mM HCl.

a) What is the pH of the buffer?

b) What would the pH be if we had added 2.8 mM of HCl instead of the amount of HCl in (a)?

c) What would the pH be if we had added 1.00 mL of 0.010 M NaOH to the original amounts in

(a)?

d) Could you use a Tris buffer system to prepare a buffer at pH 7.00? Why or why not?

Calculate the mass of Tris Base required to make 100 mL of 0.1 M pH8.5 Tris Buffer

scarletmallard305

Buffer Calculations Practice (how much to mix?)

PART A:

Make a 300 ml solution of 100mM Tris base in H₂O. CALCUALTE

Use the henderson hasselbach to make a 50 ml 50mM Tris solution at pH 8.0 by mixing Tris HCl and Tris base powders ( use formula weights of Tris-HCl and Tris base). CALCULATE How many grams of Tris HCl and Tris base did you add to make your solution?

PART B: HOW DO YOU MAKE THE FOLLOWING BUFFER; CALCULATE

Buffer: 10X TAE

0.4M Tris base

0.01M disodium EDTA

0.2M acetic acid

Make 500ml of this solution from chemical stocks. The pH of this solution should be between 8.2 and 8.3.

THEN Make a 100 ml 1X dilution of TAE from this 10X stock. CALCULATE

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Solution Chemistry, please show, explain work!!â Calculate the amount (in mg) of Trizma Base (molar Mass 121.1) required to make 50 mL OF 50.0 micro Moles Tris buffer.

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Solution Chemistry, please show, explain work!!â Calculate the amount (in mg) of Trizma Base (molar Mass 121.1) required to make 50 mL OF 50.0 micro Moles Tris buffer.

Unlock all answers

Related textbook solutions

Basic Chemistry

Principles of Chemistry Molecular Approach

Principles of Chemistry Molecular Approach

Chemistry: Structure and Properties

Chemistry: A Molecular Approach

Chemistry: A Molecular Approach

Principles of Chemistry: A Molecular Approach

Chemistry: The Central Science

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Solution Chemistry, please show, explain work!!â Calculate the amount (in mg) of Trizma Base (molar Mass 121.1) required to make 50 mL OF 50.0 micro Moles Tris buffer.