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13 Dec 2019
Question
PNP (2.8 mg) (MW = 140) was dissolved in 20 mL of 0.1 M NaOH. A small aliquot of this solution (0.1 mL) was diluted with 4.9 mL of pH 8 buffer (0.1 M), and the absorbance at 415 nm was determined. If the molar absorptivity is 15,000 L/cm/mol at this wavelength and pH, and the path length is 1 cm, what absorbance reading do you expect?
Please help me with this question and show all work
Thank you
Question
PNP (2.8 mg) (MW = 140) was dissolved in 20 mL of 0.1 M NaOH. A small aliquot of this solution (0.1 mL) was diluted with 4.9 mL of pH 8 buffer (0.1 M), and the absorbance at 415 nm was determined. If the molar absorptivity is 15,000 L/cm/mol at this wavelength and pH, and the path length is 1 cm, what absorbance reading do you expect?
Please help me with this question and show all work
Thank you
Jean KeelingLv2
17 Dec 2019