Question

Can you explain how we get the initial and final volumes for the 6 solutions?

Part A. Prepare the standard curve for Blue #1. I. Obtain about 25 mL the 1.8 × 104 M stock solution of Blue #1 in a beaker. 2. Obtain the volumetric pipets - one each of 5.00 mL, 10.00 mL, 20.00 mL and 50.00 mL sizes. Obtain the volumetric flasks - one each of 25.00 mL and 100 mL size. Find caps or stoppers that fit each flask before using them. Rinse all the glassware at least twice with DI water. 3. Set out the largest beaker for "waste" rinses, Set out six clean, dry beakers (any available sizes) to hold each diluted solution as it is made. 4. Summary of dilutions suggested by the head researcher Soluton ID Volume of inital solution meauredFinal volume of dilution Concentration of (volumetric flask) diluted solution, M (volumetric pipet) 10.00 mL of stock solution 5.00 mL of stock solution 20.00 mL of #2 50.00 mL of #2 50.00 mL of #4 50.00 mL of #5 number 100 mL 100 mL 25 mL 100 mL 100 mL 100 mL 1.8 × 10-5 M 9.0 x 106 M 7.2 x 106 M 4.5 x 106 M 2.3× 10-6 M 1.1 x 106 M #1 #2 #3 #4 #5 #6 Prepare solution #1. 10.00 mL volumetric pipet with a small portion of the Blue #1 stock solution and drain Rinse the the rinse into the large waste beaker. Pipet exactly 10.00 mL of the stock solution into the 100 mL volumetric flask. Remember to let the pipet drain by gravity. Never blow out the last drop in the tip. a. b.