Create a flow chart outlining the steps and separations followed in this experiment.
INSTRUCTIONS A. SETTING UP THE ExPERIMENT 1. Weigh out approximately 0.25 g 5-nitrobenzisoxazole in igh out approximately 0.25 g 5-nitrobenzisoxazole into a 125 mL. Erlenmeyer flask using a top-loading balance. Avoid spillage and waste as there is limited starting material available. 2. To the Erlenmeyer flask, add 1.5 ml, ethanol [CAUTION: flammable liquid, no open flames], 1.5 ml. distilled water and 7.5 mL. 2 M NaOH in the fume hood. [CAUTION corrosive, wear gloves] . Add a stir bar to the Erlenmeyer fask and use a magnetic stir plate to stir the reaction at roorm 3. Add a stir bar to the Erlenmeyer flask and use a magnetic stir plate to stir the reaction at room temperature for 15 minutes, moving the flask on the hot plate often to help dissolve the solid. B. REACTION WORK-UP 1. Using a disposable Pasteur pipette, add concentrated HCI [CAUTION: corrosive, wear gloves] to the reaction mixture in the fume hood 10 drops at a time very slowly until it reaches pH 1. Make sure to swirl the flask and check the pH using pH paper after each addition. 2. Read Lab Tips: Separatory Funnels in the Lab Manual then pour the acidified solution into a 125 ml, separatory funnel in the fume hood in your assigned space. 3. Perform liquid-liquid extractions of the aqueous layer with three 5 mL portions of ethyl acetate, each time rinsing the Erlenmeyer flask with the ethyl acetate before adding to the separatory funnel and collecting the ethyl acetate layer in a clean, dry 125 mL. Erlenmeyer flask CAUTION: pressure build-up; vent frequentlyl. , Caronna, G.; Palazzo, S. Caç½Chim 111. 1959, 89, 1009. 34