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11 Nov 2019
a) Describe in detail how you would prepare a 1-liter solution of 100 mM sodium phosphate buffer with a pH of 8.0 using 6 M HCl? The pKa value of sodium phosphate is approximately 7.2. The MW of dibasic sodium phosphate Na2HPO4 is 141.96 g/mol.
b) Calculate the final concentration of monobasic sodium phosphate, NaH2PO4 (conjugate acid), once youâve prepared the buffer. 3.
a) Describe in detail how you would prepare a 1-liter solution of 100 mM sodium phosphate buffer with a pH of 8.0 using 6 M HCl? The pKa value of sodium phosphate is approximately 7.2. The MW of dibasic sodium phosphate Na2HPO4 is 141.96 g/mol.
b) Calculate the final concentration of monobasic sodium phosphate, NaH2PO4 (conjugate acid), once youâve prepared the buffer. 3.
Keith LeannonLv2
14 Aug 2019