Question

TPE isa commonly used buffer for DNA electrophoresis. This buffercontains 89 mM Tris, 23 mM Phosphate, 2 mM EDTA as the final concentrations. The final pHis 8.3. The 10X stock solution (10 X the final concentration) isnormally prepared. You have the Tris powder (mol. wt. 121.14),Phosphoric acid H₃PO₄ (liquid 91.3 % pure,density 1.61 g/mL, mol. wt. 98) and a 0.5 M EDTA stock solution.You need to make up 1 L of 10X TPE. How would you make itup?