In an experiment, a solution of pH 4.0 containing 100 mM K+ and 0.1 mM Ca++ is required. Choose the correct way to prepare this solution
from the following options.
a. Dissolve 3.73 g of KCl in 90 ml citrate buffer of pH 4.0, add 1 ml of 10 mM CaCl2, and make up the volume to 500 ml.
b. Dissolve 0.373 g of KCl in 40 ml distilled water.Add 0.5 ml of 10 mM CaCl2 to 5 ml distilled water. Mix both the solutions and adjust the pH to 4.0 with HCl. Make up the volume to 50 ml.
c. Dissolve 7.46 g of KCl in 50 ml distilled water. To this, add 10 ml of 10 mM CaCl2. Adjust the pH to 4.0 with HCl and make up the volume to 100 ml.
d. Dissolve 0.746 g of KCl in 100 ml of distilled water, add 1 ml of 10 mM CaCl2, and adjust the pH to 4.0 with HCl.
Please explain how did you come up with that answer along with the calculation. Thanks
In an experiment, a solution of pH 4.0 containing 100 mM K+ and 0.1 mM Ca++ is required. Choose the correct way to prepare this solution
from the following options.
a. Dissolve 3.73 g of KCl in 90 ml citrate buffer of pH 4.0, add 1 ml of 10 mM CaCl2, and make up the volume to 500 ml.
b. Dissolve 0.373 g of KCl in 40 ml distilled water.Add 0.5 ml of 10 mM CaCl2 to 5 ml distilled water. Mix both the solutions and adjust the pH to 4.0 with HCl. Make up the volume to 50 ml.
c. Dissolve 7.46 g of KCl in 50 ml distilled water. To this, add 10 ml of 10 mM CaCl2. Adjust the pH to 4.0 with HCl and make up the volume to 100 ml.
d. Dissolve 0.746 g of KCl in 100 ml of distilled water, add 1 ml of 10 mM CaCl2, and adjust the pH to 4.0 with HCl.
Please explain how did you come up with that answer along with the calculation. Thanks
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