NUTRITION Chapter Notes - Chapter 14: Pulsed-Field Gel Electrophoresis, Aspergillus Flavus, Gel Electrophoresis Of Nucleic Acids

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amigo

7 Jan 2022

School

Kerala University of Health Sciences - KUHS

Department

Public Health

Course

NUTRITION

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The classic example is picnickers vomiting 4 to 6 hours after consuming salad contaminated with staphylococcus aureus enterotoxin. The consumption of s. aureus cells is not necessary, as toxins produced in the food are sufficient to cause illness. Some fungus-derived toxins have more insidious health effects. The most potent are the fungal carcinogens aflatoxins and fumonisins. Aflatoxins are produced by aspergillus flavus and a. parasitises. Besides their importance in grains, aflatoxins have also been found in beer, cocoa, raisins, peanut butter, and soybean meat. The cdc oversees a program called pulse net for the early detection of food-borne pathogens, particularly during an outbreak. The basis for this program is the use of pulsed field gel electrophoresis (pfge) under carefully controlled and duplicated conditions. Pfge generates a strain-specific dna electrophoresis pattern, or fingerprint, for each bacterial pathogen pfge is similar to standard dna gel electrophoresis but is capable of separating very large fragments of dna.

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1) What clinical manifestation do Staphylococcus aureus and Staphylococcus epidermidis share? A) nosocomial infections B) protein A antiphagocytic factor C) hyaluronidase enzyme D) enterotoxins E) staphylokinase 2) Which of the following has been historically classified as Gram-negative bacteria but is genetically more similar to low G + C Gram-positive bacteria? A) mycoplasmas B) Mycobacterium C) Clostridium D) Propionibacterium E) Nocardia 3) What is one virulence factor that differentiates Staphylococcus aureus from other species of staphylococci? A) It has a capsule. B) It can produce coagulase. C) It produces catalase. D) It can live on the surface of the skin and in cutaneous oil glands. E) It produces lipase. 4) Over 90% of Staphylococcus aureus isolates are penicillin-resistant. Why? A) They have loosely organized polysaccharide slime layers. B) They produce coagulase. C) Cell division occurs in successively different planes, and the daughter cells remain attached to one another. D) They produce Î²-lactamase. E) They produce staphylokinase, which dissolves fibrin threads in blood clots. 5) A woman comes to the emergency department with fever and vomiting. She soon develops a red rash all over her body, and her blood pressure begins to drop. What is one possible diagnosis? A) erysipelas C) scarlet fever B) endocarditis D) toxic shock syndrome E) staphylococcal food poisoning 6) Bacteria collected from a severely inflamed wound are sent to the lab for analysis. The results come back as follows: Gram-positive cocci in irregular clusters, kinase and coagulase positive, and able to grow in the presence of most antibiotics except vancomycin. The bacteria in the wound are most likely _______ A) Enterococcus. C) Staphylococcus aureus. B) Staphylococcus epidermidis. D) methicillin-resistant Staphylococcus aureus (MRSA). E) Streptococcus pyogenes (group A streptococcus). 7) Which of the following statements about "flesh-eating" streptococci is FALSE? A) It is caused by a group A streptococcus. B) It is also known as necrotizing fasciitis because it travels along the fascia. C) It causes death in over 50% of cases. D) It is considered a common complication of pyoderma. E) It involves toxemia. 8) Streptococcus agalactiae is associated with which of the following diseases? A) neonatal bacteremia B) neonatal meningitis C) neonatal pneumonia D) both neonatal bacteremia and neonatal meningitis E) neonatal bacteremia, neonatal meningitis, and neonatal pneumonia 9) What differentiates virulent strains of Streptococcus pneumoniae from nonvirulent strains? A) the species-specific teichoic acid present in its cell wall B) the type of Lancefield antigen it produces C) the presence of a polysaccharide capsule that protects it from digestion after endocytosis D) the type of toxins it produces E) the extent of the hemolytic zone it produces when it is grown on blood agar 10) Otitis media may lead to dangerous meningitis in children due to infection with _____ A) Streptococcus agalactiae. B) Streptococcus pneumoniae. C) Streptococcus mutans. D) Enterococcus. E) Mycoplasma pneumoniae. 11) Which of the following streptococci is associated with dental caries? A) viridans streptococci B) Streptococcus pyogenes C) Streptococcus pneumoniae D) Streptococcus equisimilis E) Streptococcus arginosus 12) Anthrax, which means "charcoal" in Greek, derives its name from _____ A) the airborne endospores it produces. B) the staining properties of the bacillus under the microscope. C) the black eschars it produces on human skin. D) its ability to invade the bloodstream and produce toxemia. E) the high mortality it causes in infected individuals. 13) Which of the following bacteria produce one of the most deadly bacterial toxins known? A) Clostridium perfringens C) Clostridium tetani B) Clostridium difficile D) Clostridium botulinum E) Corynebacterium species 14) Which of the following bacteria can cause life-threatening pseudomembranous colitis? A) Clostridium difficile B) Staphylococcus aureus C) Streptococcus pyogenes D) Enterococcus species E) Clostridium botulinum 15) How does the toxin from Clostridium tetani produce its action? A) It fuses irreversibly to neuronal cytoplasmic membranes, blocking release of acetylcholine at synaptic clefts. B) The smaller polypeptide of its toxin can block the release of inhibitory neurotransmitters by inhibitory neurons in the central nervous system, causing simultaneous contraction of both muscles in an antagonistic pair. C) It is a pyrogenic toxin, which triggers a diffused rash and, later, sloughing of skin. D) It destroys tissues, including muscle and fat. E) It produces antibodies that attack the neuromuscular junctions in skeletal muscle and prevent them from relaxing. 16) Listeria virulence is directly related to its ability to ______ A) produce powerful toxins. B) form very resistant endospores. C) live within cells and thus avoid exposure to the immune system of its host. D) easily become a pathogen in humans. E) resist most antimicrobial agents. 17) The pus from an abscess in a patient's jaw contains microbes that form yellowish grainy masses and appear filamentous under the microscope. Neither antifungal medication nor a normal course of antibiotics has been effective in treating the infection. The abscess is likely the result of infection with A) Actinomyces. B) Mycobacterium. C) Nocardia. D) Propionibacterium. E) Streptococcus. 18) What is the most common disease caused by Propionibacterium? A) acne B) food poisoning C) pneumonitis D) folliculitis 19) Streptococcus pneumoniae is a leading cause of ______ A) acne. C) pharyngitis B) furuncles. D) primary atypical pneumonia. E) otitis media. 20) The soil bacterium Nocardia asteroides can establish opportunistic infections of the _____ A) skin C) central nervous system B) lungs D) cardiovascular system. E) skin, lungs, and central nervous system

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1. All of the following characteristics of skin help make it a good defensive barrier against pathogenic microbes EXCEPT:

Springy elasticity

Saltiness due to production of sweat from sweat glands

Acidic pH due to oily compound (sebum) metabolism

Normal microflora of skin

Constant sloughing off of old dead epithelial skin cells

2. The majority of microorganisms comprising the normal microflora inhabiting the skin surface are:

Gram-positive fungi of the single-celled yeasts

Gram-positive bacteria of the Staphylococcus, Micrococcus, Corynebacterium, and Proprionibacterium genera

Gram-negative bacteria of the Enterobacteriaceae family

Gram-negative bacteria of the Neisseria, Pseudomonas, and Salmonella genera

All of the above

3. The pus-producing skin infection (pyoderma) can be caused by:

Staphylococci

Streptococci

Corynebacteria

A combination of staphylococci, streptococci, and corynebacteria

All of these

4. Which of the following is NOT a characteristic of impetigo?

Common in children

Can be caused by Staphylococcus aureus

Can be caused by Streptococcus pyogenes

Is highly contagious

Can be caused by pseudomonads

5. Which of the following is NOT true about acne?

It is most commonly the result of male sex hormones that stimulate increased sebaceous gland growth and secretion of sebum.

Propionibacterium acnes in particular can turn a mild case of acne into a severe case by causing increased and widespread inflammation, tissue destruction, and scarring.

Common treatments are frequent cleansing of the skin and topical ointments that reduce the risk of infection.

Low doses of oral antibiotics are often prescribed to prevent bacterial infections of lesions and are without side effects.

In most cases, acne disappears or decreases in severity on its own as the body adjusts to the hormonal changes of puberty and sebaceous gland function stabilizes.

6. Which of the following often cause nosocomial infections in burn patients?

Streptococcus pyogenes

Serratia marcescens

Pseudomonas aeruginosa

Staphylococcus aureus

b and c

7. Gas gangrene is most likely associated with infection with:

Staphylococcus aureus

Clostridium perfringenes

Streptococcus pneumoniae

Neisseria gonorrhoeae

Pseudomonas aeruginosa

8. The leading cause of preventable blindness in the world is caused by:

Chlamydia trachomatis

Haemophilus influenzae

Neisseria gonorrhoeae

Staphylococcus aureus

Streptococcus pneumoniae

9. Chickenpox is a disease of chickens and is caused by the poxvirus, cowpox is a disease of cattle, and smallpox is a human disease that commonly affects only children. True or false?

10. Antibiotic is used to treat the eyes of newborn infants when:

The mother has gonorrhea

The mother has genital herpes

Neisseria gonorrhoeae is isolated from the newborn's eyes

Always

The mother has a history of multiple sex partners

11. The virus used to immunize individuals against smallpox causes the disease:

Chickenpox

Measles

Cowpox

Molluscum contagiosum

Warts

12. Which of the following fungal diseases result from an opportunistic fungal infection?

Zygomycoses

Aspergillosis

Candidasis

All of the above

None of the above

13. Rheumatic fever is caused by which organism?

Corynebacterium diphtheriae

Staphylococcus epidermidis

Staphylococcus aureus

Escherichia coli

Streptococcus pyogenes

14. Which of the following components of the respiratory system is normally free of microorganisms?

Lungs

Nasal cavity

Pharynx

Trachea

Bronchi

15. Which of the following is true about diphtheria?

It is caused by strains of Corynebacterium diphtheriae infected with a prophage that carries an exotoxin-producing gene.

A pseudomembrane composed of Corynebacterium diphtheriae cells, damaged epithelial cells, fibrin, and blood cells forms that can block the airway and cause suffocation.

The exotoxin spreads throughout the body damaging multiple organ system by interfering with cellular protein synthesis.

It can be prevented with DTP (diphtheria, tetanus, and pertussis) vaccine and treated with both antitoxin and antibiotics.

All of the above are true.

coppertrout695

Staphylococcus Aureus

1. Main biological features

a. Cell shape, arrangement, Gram stain, endospores, capsule, unusual features.

2. Clinical Manifestations

a. List the diseases that are produced by this microorganism.

3. Mechanism of Disease

a. List of how the microorganism induces disease: Toxin, proteolysis, pyogenic, immunological mechanism or multiple mechanisms.

4. Transmission

a. How the microorganism is transmitted and who is at risk of acquiring the infection.

5. Prevention

a. List the most common prevention methods

b. Is there a vaccine?

6. Treatment

a. Is antimicrobial intervention necessary?

b. Have resistant strains been detected?

c. List the common methods of treatment (you donât need to list drug names).

d. Prognosis â Mortality associated with the disease

NUTRITION Chapter Notes - Chapter 14: Pulsed-Field Gel Electrophoresis, Aspergillus Flavus, Gel Electrophoresis Of Nucleic Acids

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