BIOL 483 Lecture Notes - Lecture 10: H3K27Ac, Thymocyte, Nucleosome
Document Summary
Chr are important to change prevailing chromatin state - remodel chromatin to open up/ close down -> heavily impacts transcription regulation. Promoters = where rna pol sites, marked by h3k27ac, h3k4me4. Enhancers = where tf/ mediator complex sits, marked by h3k27ac, h3k4me1. When tf binds enhancer -> cause looping so that enhancer reacts with promoter to enhance gene expression -> drive rna pol to drive transcription. > malignant rhabdoid tumours = 100% have recurring mutation in subunit of swi/snf complex. Chr can act in same/ different genomic contexts. Chromatin assembly/ accessibility eg. dense heterochromatin with linker histones in hp1 -> use. Specific triggers for activities due to autoinhibitory domains/ hss domain, which engage nucleosome in different ways. Many remodelers rely on targeting by tf or different accessory proteins. Activity regulated by environment around them/ environmental & developmental cues. Factors that can regulate dna translocation = tf, non coding rna, diff nucleosomal epitopes (markers), histone modifications.
