CHEM 241L Lecture 5: Experiment5
Document Summary
Experiment 5: determination of the molecular weight of myoglobin by gel-filtration. Analytical chemistry lab 241l, section 403, room 401. I pledge that i have not used someone else"s old or current lab report when writing this lab report. I pledge that i did not collaborate with any other students and that the report i submitted here contains my own ideas, thoughts, observations, calculations, data, conclusions and answers. Lastly, i pledge that the data presented in this report was collected by my lab partner and myself during my scheduled lab period . Molecular weight and elution volumes for all samples analyzed. Vm is the total volume of the mobile phase. Dextran blue is above the fractionation range of the sephadex g-75 so its elution. Phenol red is below the fractionation range of sephadex g-75 so its elution volume =vm=56. 0ml. So when calculating k for hemoglobin separated by sephadex g-75 the vr obtained is 17. 0ml, vm is 56. 0ml and vo is 14. 0ml.
