BIOLOGY 207 Lecture Notes - Lecture 11: Interactive Media, Exponential Growth, Passive Transport
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Dead cells uoresce red because propidium can only enter dead cells. Live cells uoresce green because syto-9 enters all cells. Can count number of live and number of dead cells. Flow cytometry and cell-sorting: direct counting of living and dead cells. Light scatter correlates to cell size and granularity. Fluorescence measures live and dead cell content. The data can then be graphed to quantify the two populations. We have alive and dead cells stain that allows you to measure of red uorescence. Red uorescence intensity is higher in this cell population which represents dead cells, And here are the cells that have a low red uorescence intensity, which means that they are the living cell population. Concept check \what is the fastest way to measure cell density: petroff-hausser counting chamber, optical density, streak plate method, flow cytometry. Most bacteria divide by binary ssion, where one parent cell splits into two equal daughter cells.
