MCB 300 Lecture Notes - Lecture 15: Hydrolysis, Denitrification, Chemiosmosis

• School: University of Illinois
• Department: Molecular and Cell Biology
• Course: MCB 300
• Professor: C Vanderpool

Document Summary

Reactions in glycolysis, tca cycle, and fermentation that couple breaking of. High-energy bonds in intermediates to formation of atp from adp and pi. All these pathways produce reduced cofactors, which are temp storage sites for electrons, which will then enter etc and generate energy. Coupling oxidation of reduced co-factors to synthesis of atp through respiration. Energy stored in membrane can drive atp synthesis via atp synthesis. Energy storage as membrane potential - pmf (h+ gradient) Pmf used for energy-requiring processes (atp synthesis, substrate import, flagellar rotation) Energy released form e transport is stored as membrane potential (pmf) and this force is used for energy required processes like biosynthetic reactions, importing substrates, making atp, flagella rotation. Electron transfer from a metabolite to a molecule supplied in the medium e. g. , nadho2 for aerobic respiration e. g. , nadh no3- for anaerobic (nitrate) respiration. Energy becomes available because affinity of electron acceptor for electrons greater than that of nadh.