MCB 150 Lecture Notes - Lecture 26: Translocon, Protease, Endoplasmic Reticulum

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Proteins that stay in and functioning in the cytoplasm have no additional target signals . Proteins destined for the nuc has an nls. Where a protein is translated could limit where that protein ends up. Protein is either left alone, or put onto the er. Becomes membrane bound ribo if on er. If no signal, it is translated on a free ribo in cytoplasm. Can end up in the nuc, mitoch, peroxisome, or chloroplast. Could go to golgi, secretory vesicle, endosome, lysosome, or plasma membrane. Imp: protein location depends on where it was made. Premise: mark a molecule in a way that it can be detected but nothing else can, Pre- pulse you can see where it originates and where it goes. Took living cells (making proteins) and realized that tons of proteins are being made. Giving cells radioactive version of a protein to be detected. Hot cold (labeled/ unlabeled in this context)

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