CBIO 3400 Lecture Notes - Lecture 7: Small Gtpase, Oligosaccharide, Translocon
8 Mar 2019
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Document Summary
N-linked glycosylation: modification of the n-glycan continues in the lumen in the golgi body, n-linked glycosylation is also functions in the golgi. Oligosaccharide processing: transfer of large precursor, removal of 3 glucose and one mannose to get out of er and go to golgi. Trying to figure out which protein is acting as a translocon in the er membrane. Suspected sec61, sec62, and sec63 could be the translocon: why was it important that they removed the stop codon on the mrna for alphaf, don"t want to release the polypeptide. If there"s a stop codon, release factors will come in and cleave the polypeptide. Crosslinking experiment: explain the data in lanes 5 and 6. In lane 5, alpha f is present, so crosslinking is occurring between alpha-f and the translocon. Lane 6 shows us you can get a change in mobility via using endo h.
