BISC403 Lecture Notes - Lecture 22: Genetically Modified Crops, Microinjection, Knockout Mouse
18 May 2017
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Document Summary
If you"re trying to examine a mutant phenotype, might not know which of two genes; you know mutation somewhere in a range; role of transgenes cloning a gene into a vector and expressing that. Transgenes can connect genes with phenotypes: can use to determine which transgene rescues, so that you know which gives the mutant phenotype. Genetically modified crops are widely used: for fast growth; example salmon go from young to adult in very short amount of time. Knockins introduce specific mutations in specific genes: a knockin is a variation on a knockout, the gene is altered, not deleted. Knockins can be used to make models of human disease: mouse model for achondroplasia was constructed to contain the same mutation in the fgfr3 gene as seen in the human gene, dwarf mouse. Reverse genetics: select a gene; generate a null mutant of the gene; phenotype.
