CHEM 143A Lecture Notes - Lecture 1: Lab Notebook, Graduated Cylinder, Pipette

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ceruleantortoise124

8 Sep 2015

School

UCSD

Department

Chemistry and Biochemistry

Course

CHEM 143A

Professor

Bob Ternansky

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Document Summary

This form is to be used for the purposes of planning your laboratory activities. It allows you to organize yourself before entering the lab and helps you to work in an efficient and safe manner. You should use your own words in constructing this document but there should be enough detail to allow you to follow the steps without referring to the original experimental procedure. As you perform the various operations, describe your actual activities in your laboratory notebook. You are required to have this prepared at the beginning of each laboratory period and to turn it in to your ta after the experiment is complete. A brief sample is shown below followed by a blank form for your use this quarter. The pre-lab does not go into the laboratory notebook. You may fill this out on a computer and print it for use in the laboratory.

Related Questions

I am not sure what 1,3,5 is asking. Can you show me how you gotyour answer and draw the picture?

And please open this picture in a new window to see it better.Somehow my picture looks abnormal.

Thank you for your help in advance.

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calculate the amount of the major component in the eucalyptus oil and the amount of maleic anhydride you will need to use in the diels alder reaction.

JBE/KWK CHEM 222 Safety Issues Caution! Avoid contact with and do not breathe the dust of the maleic anhydride! use these reagents in a hood or under a fume extractor Procedure Spring 2018 away from flames and Analysis of Eucalyptus Oil by GC-MS-A sample 1 Î¼ãof the eucalyptus oil was injected into the GC- MS. Use the gas chromatograph and peak integration data provided on BlackBoard to calculate the percentage of all peaks produced from the eucalyptus oil. The sharp peak with a very low retention time is most likely acetone and not a component of the oil, and therefore was not integrated. Record the peak areas in your lab notebook and show your calculation for percent of the major component (largest peak area) in the oil Diels-Alder Reaction- Calculate the mass of the unknown CioHthe diene in 0.50 g eucalyptus oil ng the GC data (the percent composition of the largest peak of the eucalyptus oil) assuming that the peak areas are proportional to the component masses. The mass you calculate shoulc be entered into the synthesis data chart in your laboratory notebook. The diene and dienophile (maleic anhydride) are added to the reaction in equal molar quantities Calculate the moles of diene present in the 0.50 g eucalyptus oil and calculate the mass of maleic anhydride that you will add to the reaction All glassware must be clean and dry throughout the procedure as the maleic anhydride the Diels-Alder adduct are readily hydrolyzed by water and In a clean, dry microwave reaction vessel equipped with a stir bar dissolve 0.5 g eucalyptus oil in 5 mL THF and add the calculated mass of maleic anhydride. Cap the reaction vessel tightly and put into the microwave carrousel and record which slot your vessel is in. After the completion of the reaction using the microwave reactor let the vessel cool to room temperature. The actua measured mass of maleic anhydride should be entered into the synthesis data chart in your laboratory notebook. While your reaction is being heated, calculate the actual mass of the diene in your reaction based on the measured mass of oil in your flask and enter this value in the synthesis data chart and calculate the limiting reactant in your reaction If no crystals have formed by the time it is cooled to room temperature, create a nucleation site dipping a stirring rod into the solution, withdrawing it, allowing the ether to evaporate, then swirl the stirring rod in the solution. When the crystal formation appears to be complete, cool the flask in an ice water bath for an additional 5 minutes. Collect the product by vacuum filtration washing the crystals with 5 mL cold petroleum ether (place your flask with the petroleum ether in the ice water bath to cool while your crystals are forming) Recrystallize the Diels-Alder adduct from methanol in a small lask using a steam bath for a heat source. Just as the product can undergo hydrolysis with water, it can go through solvolysis with methanol. Avoid boiling the solution for extended periods of time. Once the product has been recrystallized and collected by vacuum filtration, transfer the crystals to a pre-weighed watch

Good Afternoon,

I am doing a laboratory report for the course entitled: Principles of Instrumental Analysis. It is a 4th year course and i have been tasked with the following analytical problem, "Determine the DEET composition in common bug sprays." In other words, I have to determine the concentration of DEET in a couple of bug sprays. The sprays and DEET are supplied. Secondly, I have to prepare a protocol for measuring the concentration of DEET in my samples and a GC:MS method for analysis of DEET. By the way, GC:MS is short for 'Gas chromatographyâmass spectrometry' and as you may know it is an analytical method that combines the features of gas-chromatography and mass spectrometry to identify different substances within a test sample. Basically, I need your help in giving me a layed-out protocol (such as we would see in a manual) of what I will do in the lab to answer the above analytical problem. You can think of it as a lab manual that you would write so that someone else could go in the lab and perform the experiment in your place.

It should have all of these components.

1. Brief (!) introduction of the problem (1 short paragraph)

2. Sampling â how much (be precise), in what form, how many, where, etc.

3. Sample preparation â list all reagents needed up front. Then list all steps and procedures you will use to prepare the samples for instrumental analysis. You should know what concentration ranges you are aiming for.

4. Standards preparation â list steps involved in preparing your standards used for calibration (if you need to do this).

5. Instrumental Analysis â list the procedure for running the instrument, including any blanks, standards. The sequence of runs (sample, standard, blank, etc) should be listed.

6. Data Analysis â how will you go from the raw data provided by the instrument to a value with a Â± deviation on it.

Please refer to the above question. Can you give me a detailed layout of how I will determine the concentration of DEET in my samples before doing the GC:MS analysis? You do not need to determine the composition or synthesize DEET. You need to determine the concentration of DEET, in a couple of bug sprays. The sprays and DEET are supplied.

I'm not quite understanding. Thank you for your clarification once again.

CHEM 143A Lecture Notes - Lecture 1: Lab Notebook, Graduated Cylinder, Pipette

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