BIOL 1107 Lecture Notes - Lecture 2: Optical Microscope, Tonicity, Prokaryote
Document Summary
The shape and chemical properties of a biological molecule are dictated by the functional group it contains. Biomolecules are hydrophilic or hydrophobic, form a color due to reaction with reagent, separate based on physical properties, or absorb specific wavelengths of light. Amino acid"s r-group determines the shape and function of the protein. Samples move at various rates depending on their degree of adsorption to the matrix and solubility in the solvent. Tests presence of proteins and small peptides. Color and intensity based on the type and amount of protein present. Graph what was transmitted (wavelength) vs. how much was absorbed. Diffusion = random movement of molecules due to a concentration gradient. Osmosis = diffusion of water across a cell membrane. Tonicity = the effects of osmosis on the cell. To predict the direction of movement of water molecules and non- Parfocal = image stays nearly in focus changing from one objective lens to another.
