ANSC 1001 Lecture Notes - Lecture 3: Xenotransplantation, Cottonseed Oil, Cell Potency
Document Summary
What is biotechnology: practical use of biological processes. The use of biological processes or living microorganisms in industrial production: early examples include. Making beer: later, more hi-tech examples include. Research in dr. tian"s lab: transgenic animals, gmos, somatic cell nuclear transfer. Cloning: embryonic stem cells (esc, induced pluripotent stem cells, molecular embryology, epigenetics. Transgenic organisms: an organism with a foreign gene inserted in the genome. Artificial means: transfer of injected egg into surrogate mothers, screen offspring for expression of transgene, transgene is hopefully inherited in a mendelian fashion. Methods for transgenic organism generation: direct microinjection, cloning using transfected cells, retroviral vectors or sperm vectors, embryonic stem cells. Transgenic applications: bioreactors (insulin, organ, cell, tissue donors, basic research & disease model, agricultural applications. At least 90% of us corn, soybeans, cotton are transgenic. + cottonseed oil is found in lots of food. Up to 75% of processed foods in supermarkets contain genetically engineered ingredients.