MIMG 102 Lecture Notes - Lecture 20: Southern Blot, Dna Replication, Sv40
20 Mar 2019
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Document Summary
Dna viruses 3: viral dna is present in transformed cells. Viral genome must integrate into the host. Rare; happens by chance; non-obligated part of viral life cycle: first important discovery about mechanism of oncogenic transformation: transformed cells contain viral dna integrated into their genome. Discovered by southern blot of isolated genomes of transformed cells: experiment: Infect mouse cells with sv40, cells grow on top of each other (why mouse cells?) Pick foci (they are transformed cells) & control southern blot. Digest with restriction enzyme to generate fragments. Probe membrane & look for viral signal. Transformed cells always contain parts of viral dna in their genome: but integration of the viral genome is rare; only a portion of genome is integrated (which part?) Virus push cell to s phase -> dna replication. Viruses don"t grow in cells that don"t replicate. Function of viral early proteins: stimulate cell cycle -> viral replicate. Progeny virus released & infection spread: lytic or non-lytic.
