LIFESCI 3 Lecture Notes - Lecture 15: Internal Ribosome Entry Site, Kozak Consensus Sequence, Start Codon
Document Summary
Termination: overview, very similar in prokaryotes & eukaryotes, encounter stop codon in the a site, need many factors to signal to the ribosome to stop translation & disassemble, will set it up to reassemble for new translation. Knocks trnas off the ribosome"s e & p sites: if3 binds e site, helps dissociate small and large ribosomal subunits from mrna. Antibiotics: many target prokaryotic ribosomes, antibiotic puromycin binds in a site, peptidyl transferase tries adding a polypeptide chain onto it abortion of translation protein becomes nonfunctional eventually the organism dies because it can"t continue dividing. Q: you perform 2 in vitro translation experiments: one includes a wild type if2 and the other includes a mutant version of if2 that cannot hydrolyze gtp. Which of these centrifugation results (a = highest peak is 70s; b = 2 peaks @ 50s & A: b: don"t get full assembly of large subunit when there"s a mutation in if2.
