PCB 3233 Lecture Notes - Lecture 3: Immunoglobulin Class Switching, Rna Splicing, Rag1
2 Apr 2020
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Immature b-cells only show igm on their surface. Mature b-cells show igm and igd on their surface. 2- negative selection: testing bcr to assure it does not bind to your own health cells, apoptosis if cell is not appropriate. 3- positive selection: surviving b-cell wins, final maturation. Aid is the enzyme for somatic hypermutation and isotype switching. Rag1 & rag2 is the enzyme for somatic recombination. B-cells move from the bone marrow to the blood to secondary lymphoid. Transcription and translation will take place all the way up to the large pre-b cell. Surrogate light chain is used during the large pre-b cell stage to see if the heavy chain is functional. If yes, the surrogate light chain can bind. Ig and ig must signal the bcr as functional. Pre-b cell receptors lead to intracellular signals that halt rearrangement at ig heavy-chain locus = allelic exclusion. Vpreb and 5 make up the surrogate light chain.
