BIO-0046 Lecture Notes - Lecture 4: Polyacrylamide Gel Electrophoresis, G Protein, Protein Dimer
Document Summary
This is a cartoon image of her2"s (erbb2) structure. It is in the extended conformation, which is generally more active. In the absence of ligand, domain ii/iv interact with each. In her 2, no domains are interacting, it"s in the extended conformation. Domain ii is always open/accessible- but that doesn"t mean the kinase domain is always active. It will dimerize with other ligand-bound her/erbb family o o other members. People with lots of her2 don"t do well with normal cancer treatment. Block domain 2 (dimerization domain) because as soon as you dimerize, you can get allosteric activation. Inhibit kinase domain because even if it does dimerize, if the kinase domain doesn"t work it won"t trans-autophosphorylate and become active. Proteins that are normally produced by b cells. In the presence of intact cells, antibodies remain extracellular (too large) You are studying the tyrosines in the tails of various rtks under different conditions.
