01:119:115 Lecture Notes - Lecture 8: Pyruvate Dehydrogenase, Inner Mitochondrial Membrane, Acetyl-Coa

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3 Oct 2018
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I. Intro to cell respiration
A. Overview of eukaryotic respiration
1. C6H12O6 +6O2 → 6CO2 6H2O + Energy (ATP)
a) Redox reactions follow the H
(1) What is oxidized? = Sugar
(2) What is reduced? = Oxygen
(a) Transfer of e- from sugar to O2
2. NAD+ + H = NADH
a) NADH = stored energy that will be used to make ATP
3. Energy in ATP is stored in the last phosphate group. It is spring
loaded, meaning it pops off really easily
II. Stages of cell respiration
A. Glycolysis
1. Where? - Cytosol of cell
2. Function? - Convert 1 glucose to 2 pyruvates and 2 net ATP
3. Glucose enters the cell via facilitated diffusion by GLUT1
(1) No oxygen required… Anaerobic
4. Stage 1: Energy Investment Phase
a) Takes 2 ATP to work (endergonic)
b) ΔG is positive (not spontaneous)
c) 1 glucose converted to 2 G3P (glyceraldehyde 3-phosphate)
5. Stage 2: Energy Payoff Phase
a) Energy payoff phase (exergonic)
b) ΔG is negative (spontaneous)
c) 2 G3P converted to 2 pyruvate, 2 NADH and 4 ATP made
6. -2 (go in) + 4( comes out) = 2 net ATP from glycolysis
B. Oxidation of Pyruvate to Acetyl CoA
1. Where? - Entering mitochondria
2. Function? - Converts pyruvate into Acetyl CoA
3. Outer mitochondrial membrane - Pyruvate diffusion through small pores
(passive)
4. Inner mitochondrial membrane - Pyruvate is taken by carrier protein
(active)
5. Each pyruvate converted to Acetyl Coenzyme A
6. It is then catalyzed by pyruvate dehydrogenase
a) Pyruvate dehydrogenase - removes H+ and builds NADH
b) NO ATP
C. The Citric Acid Cycle
1. Where? - inside cytosol of mitochondria (mitochondrial matrix)
2. Function? - breaks down Acetyl CoA as efficiently as possible
3. Goal? - build electron acceptors to give to electron transport chain
4. 1 glucose = 2 turns (1 turn per acetyl CoA)
5. Steps:
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