BIOL 230W Lecture Notes - Lecture 29: Occludin, Cadherin, Tight Junction
Document Summary
Rdf motif of fibronectin: rgd motif accessible when fibronectin is in an inactive form, fn to fn binding motifs not accessible in inactive form, don"t want ecm inside of the cell. Binding of integrin to fibronectin causes the integrins to become clustered in the plasma membrane: concentrates fibronectin to promote more fibril interactions, positive feedback mechanism. Mutated src protein is always bound to fak despite phosphorylation regulation in some cancers: overactive src causes mechanism to occur unregulated, affects cancer development. Actin: protein polymer made of many repeating monomers. If actin was depolymerized, it could not fully activate fibronectin. Fak: bridge activity of ecm and cytoskeleton: kinase. If fak is not working, fibronectin would not form fibrils. Intracellular organization of cytoskeleton: modified cell motility. Increased active fibronectins at the integrin cluster promote further interactions and fibril assembly. Cells attach to each other via proteins. Occluding junctions (tight junctions: work to seal cells together, causes molecules to travel through the cell.
