BMCB 658 Lecture Notes - Lecture 11: Phospholipid, Carboxyglutamic Acid, Gla Domain
19 Aug 2016
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Two types of subunits: catalytic trimer. Experimentally separate the catalytic portion: catalysis goes forward like a non-allosteric enzyme (hyperbolic curve, ctp has no inhibitory control. Phosphate can convert an inactive precursor into an active enzyme (or vice versa) Example: glycogen phosphorylase: glycogen: polymers of glucose, storage in animals, glycogen phosphorylase: enzyme in carbohydrate metabolism. Two forms: phosphorylase a & b: phosphorylase a: More active form (breaks down glycogen: phosphorylase b: The two forms respond to different allosteric effectors this depends on tissue. Example: sodium potassium pump: transports sodium across membrane out of the cell, potassium is transported into the cell, atp donates p to aspartate 369 on enzyme, conformation change in enzyme. 1: one forms binds to sodium, the other binds to potassium. Phosphorylated form: binds 3 na+ and moves them out. Dephosphorylated form: binds 2 k+ and brings them in. Cleavage of one or more covalent bonds transforms it into the active enzyme.
