GN 301 Lecture Notes - Lecture 14: Combined Dna Index System, Kary Mullis, Microsatellite
Document Summary
10:16 am: polymerase chain reaction, kary mullis developed process, thermal cycler alters temperature to carry out all steps of pcr, process. Denature dna by heating to 95 c; strands separate: breaks h bonds. Primers anneal to identify target that will be amplified (50-65 c) E(cid:454)te(cid:374)di(cid:374)g: ta(cid:395) pol(cid:455)(cid:373)e(cid:396)ase adds (cid:374)ucleotides to (cid:1007)" e(cid:374)d of p(cid:396)i(cid:373)e(cid:396) (cid:894)7(cid:1006) c(cid:895) Repeat: electrophoresis, put samples in negative wells of gel, run current through sample and dna moves to positive end. Short tandem repeats: different alleles of same trait have different lengths due to diff. # of repeated dna sequences: useful loci for testing must be polymorphic (many forms, each band is measured in bp, electrophoresis separates pieces. Fda approved testing for 65 genetic traits in 2016: databases, kaiser permanente, uses genetic database and medical database to develop new treatments and improve medical research, members donate dna to research, fbi. Federal offendants and detained immigrants are mandated to give dna.
