MMG 301 Lecture Notes - Lecture 24: Allochthon, Human Microbiota, Genomics
3 Apr 2017
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Lecture 24: define ecosystem, ecology, community, population, and guild. Ecosystem- microbial communities that interact with communities of macroorganisms and the physiochemical environment. Ecology- study of microorganisms in their natural or managed environments. Community- mixture of different guilds conducting complementary physiological processes in a habitat interact. Population- individual cells of the same type multiply. Guild- populations of organisms doing similar metabolism: explain the difference between richness and abundance. Richness is diversity (different species) and abundance is number of each species: define allochthonous. Fluorescence in situ hybridization utilizes sequence-specific oligonucleotides that have an attached fluorescent moleulce to detect specific organism with populations: compare and contrast the community sampling and environmental genomics approaches. Community sampling is when pcr amplify 16s rrna gene and do dna sequencing, then compare with other sequences. Environmental genomics uses high-throughput sequencing methods to obtain dna sequence of genomes of all community members: describe how single gene pcr products are generated and analyzed for community sampling.
