BIOL 1208 Lecture : BIO Lab 1208 Final Notes
Document Summary
Biological catalysts which increase the rate of the reaction without being used up or altered in the reaction. Do it by lowering the activation energy only. Example: nitro glycerin (shake it and it blows up) Steps to using the spectrophotometer: set the wavelength, set the mode to transmittance, set transmittance to 0, insert blank and set transmittance to 100, remove blank and insert sample, switch mode to absorbance, read absorbance. Beer"s law: absorbance is directly to concentration, **know this graph: Scientific method: define a question, gather information and resources (observe, form a hypothesis, test the hypothesis by doing an experiment and collecting data, analyze the data, interpret the data and draw conclusions, publish results, retest. Hypothesis: ho: null hypothesis- no difference between the control and treatment (no change, ha: alternative hypothesis: there will be a difference between the control and treatments.
