BIOL 1208 Lecture Notes - Lecture 5: Catechol Oxidase, Catechol, Enzyme Kinetics
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**the relationship between transmittance and absorbance is inversely proportional (for example, a 0 mm solute would be t=100%, a=0%: procedure: Set wavelength to desired number: set mode to transmittance, set transmittance to 0% (sample holder is empty, insert blank, set transmittance to 100% It is important to blank the solution because a spectrophotometer uses the relative amount of light that passes through a solution to determine its concentration. Hence, it is important to tell the machine how much light is 100%, which is what (cid:862)blanking(cid:863) does. The blank is a reference point for 100% transmittance and 0% absorbance: remove blank and insert sample, switch mode to absorbance, read absorbance, relationship between absorbance and concentration: absorbance and concentration is. The relationship between directly proportional: as concentration increases, absorbance increases and transmittance decreases, standard curve: resource used to convert the absorbance value to the concentration, concentrated tubes are the least (cid:862)see-through(cid:863).
