BIOL 2300 Lecture Notes - Lecture 3: Archaea, Acidophile, Food Spoilage

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8 Sep 2016

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Chapter 4: microbial growth: photoautotroph:sunlight, photoheterotroph: sunlight, chemolithoautotroph: inorganic, chemoorganoheterotroph: organic. Nutritional factors: required elements: c, o, h, n, s, k, magnesium, calcium, iron, organisms based on carbon use: Autotrophs: inorganic carbon (co2: trace elements: small amounts the organism needs. Growth factors: some cannot synthesize cell constituents. The elements have to be added to the organism: can display wide variety of factor requirements. Component of amino acid, lipids, nucleic acids, and sugars. Component of nucleic acids, membrane lipids, and atp. Potassium, magnesium, and calcium required for the function of certain enzymes. Bacterial growth in lab: closed batch system: no input of nutrients. Population grows in a predictable, steady growth o. Log or exponential phase: huge growth of the organism. Cells compete for resources: they grow exponentially and then compete for nutrients. Position in the colony determine the resource availability: on the edge: have little competition/ high oxygen stores, in the middle: high population density.

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Microbiology Unit 2 Applied and Environmental Assignment Part 1: Identify Nutritional requirements from Chapter 6&14 (and review from chapters 1 & 2) 1. Determine which of the 4 macromolecules require the following elements: a. Carbon: carbohydrates___ lipids___ proteins___ nucleic acids___ b. Nitrogen: carbohydrates___ lipids___ proteins___ nucleic acids___ c. Sulfur: carbohydrates___ lipids___ proteins___ nucleic acids___ 2. The essential elements mentioned above are cycled through cells and the earth in biogeochemical cycles (the carbon, nitrogen, and sulfur cycles.)Select the correct answer or fill in the blanks to summarize these cycles: a. Carbon dioxide is taken in from the atmosphere by [autotrophs/heterotrophs] and converted to glucose through carbon ________________. When heterotrophs eat autotrophs they use catabolism to break down glucose to make ATP and waste carbon is released as ___________. b. There is plenty of nitrogen available in the atmosphere but it is not accessible to organisms because it is in the form of Nitrogen gas (N2). N2 is fixed by [bacteria/plants/animals] and converted to ammonia (NH3.) NH3 can be used by [bacteria/plants/animals] to build their cells. Next, by consuming food with amino acids,[bacteria/plants/animals] can get access to nitrogen for their own cellular needs. c. There is also a good quantity of sulfur in dirt, rocks, and the atmosphere. Sulfur cycles between H2S => S0 => SO4 => SH groups in Proteins => back to H2S.Using _________________ respiration, anoxygenic photosynthesis, and chemolithoautotrophy [bacteria/plants/animals]convert H2S and S0 to SO4 which is taken in by [bacteria/plants/animals] and used to build their cells. When [bacteria/plants/animals]eat plants they take in essential amino acids containing sulfur and recycle these to make their proteins. d. _________________ break down waste and dead organisms and recycle nutrients back into the cycles. *The C and N are summarized here: https://youtu.be/2D7hZpIYlCA and https://youtu.be/leHy-Y_8nRs 3. Match the main metabolic classifications to the description: Classifications: photoautotroph/ photoheterotroph/ chemoautotroph/ chemoheterotroph o A microbe that makes its own food by taking carbon from CO2 in the atmosphere and its energy from sunlight o A microbe that makes its own food by taking carbon from CO2 in the atmosphere and its energy from reduced inorganic chemicals o A microbe that eats other organisms to get organic carbon and energy o A microbe that eats other organisms to get organic carbon but gets energy from sunlight 4. Identify the phases of the bacterial growth curve and define what occurs in each phase Phase A = ______________ Definition: Phase B = ______________ Definition: Phase C = ______________ Definition: Phase D = ______________ Definition: Part 2: Unique ecosystems containing microbes 5. Watch the video that shows hydrothermal vents: https://youtu.be/RO3nO98gH_M a. Describe bacteria that live at the vents using terms from the unit 2 reading guide: ________________________________________________________________________ b. Write a 2-3 sentence reflection about hydrothermal vents and the contribution of microbes to this ecosystem: 6. Apply what you have learned to the TED-Ed video about Biofilms: https://youtu.be/pHLP5CZMnL4 a. Give the terms for cell structures mentioned in the video that we learned in Chapter 4 and 7: i. âsticky appendagesâ thatallows bacteria to attach and begin the biofilm: _______________ ii. Extracellular matrix/gooey matrix that holds cells together: ____________________________ iii. Exchange genetic information while packed together: ____________________________ iv. Communicate with each other by _________________________________________ sensing. v. Produce chemical to kill microbial competitors: ____________________________ factor.

BIOL 2300 Lecture Notes - Lecture 3: Archaea, Acidophile, Food Spoilage

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