MIC 201 Lecture Notes - Lecture 2: Eyepiece, Protozoa, Crystal Violet
10 May 2019
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Iris diaphragm: controls the amount of light going thru the specimen. Eyepiece( ocular lens): enlarges the primary image of a specimen. Objective lens: produces the primary image of the specimen. Condenser: concentrates the light from the white light source onto the specimen. Limit of resolution for a light microscope is approximately 2 m. Fungi : > 50 um, 100- 400x. Virus : < . 25 um cant see. Shapes: coccus- round, bacillus- rod shaped, spirochete: spiral shaped. Smear: put water first, then smear bacteria, then flame the slide, then put dye on it. Pure culture technique: the most common and reliable method of isolating bacterial colonies. Stains are used in order to see bacteria bc some bacteria are colorless. Negative staining: uses acidic dyes to darken the background and leaves the cells unstained. Way to see shape and size of the cell.
