ANTH 011 Lecture Notes - Lecture 28: Wilhelm Johannsen, Gel Electrophoresis Of Proteins, Biological Anthropology

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Indirect methods: concern in anthropology is to find techniques that would provide information about variation in molecular structures. Look at visible phenotype- anatomy and physiology of an organism. Immunological method- used to compare protein structure between species. Dna hybridization method- used to compare dna sequences between species. Compares the total genomic dna of 2 species to give an overall measure of. Involves making hybrid dna molecules- 1 strand from 1 species and a another strand from a different species. Dna sequencing- most widely used tool in anthropological research. Determining the actual base sequence of a gene or stretch of dna. Polymerase chain reaction (pcr)- an extraordinarily small amount of dna can be used to make millions of copies of a specific dna segment. Depends on enzyme called taq polymerase- causes the extension of a single strand of dna. Used in anthropology to study evolutionary patterns in mitochondrial dna and nuclear dna recovered from bone, or ancient dna.

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