MIP 343 Lecture Notes - Dendritic Cell, Monocyte, B Cell
Document Summary
Grow in different cell type or creature naturally attenuated. Inactivated (killed) c. diphtheria and c. tetani + pertussis toxoid subunits. Old vaccine was inactivated: pertussis side effects included seizures, elevated. Children: inactivated b cell response attenuated . Flumist (live, attenuated, infects nasal epithelium localized immune response), intradermal (live, attenuated), intramuscular (inactivated/killed) The pathogenic virus is isolated from a patient and grown in human cultured cells. The cultured virus is used to infect monkey cells. The virus acquires many mutations that allow it to grow well in monkey cells. The virus no longer grows well in human cells (it is attenuated) and can be used as a vaccine. Either mutate virulence gene or delete virulence gene. If virulence gene is deleted, the vaccine may be less immunogenic. Something used to activate the danger signals from a vaccine. Old, used for decades before finding how it works. Oil-in-water emulsion with muramyldidpeptide (mdp), a constituent of mycobacteria.