LIFE 102 Lecture Notes - Lecture 48: Sanger Sequencing, Craig Venter, Human Genome Project
Document Summary
The study of whole sets of genes and their interactions. The application of computational methods to the storage and analysis of biological data. *two approaches complemented each other in obtaining the complete sequence. *the initial approach built on an earlier store house of human genetic information. *then, j. craig venter set up a company to sequence the entire genome using an alternative whole -genome shotgun approach. *this used cloning and sequencing of fragments of randomly cut dna followed by assembly into a single continuous sequence. Steps: cut the dna into overlapping fragments short enough for sequencing, clone the fragments in plasmid or other vectors, sequence each fragment, order the sequences into one overall sequence with computer software. *software is available that allows online visitors to search genbank for matches to: Common stretches of amino acids in a protein. *the human genome project fostered development of faster less expensive sequencing techniques.