MICR-4160 Lecture Notes - Lecture 2: Bacillus Subtilis, Deoxyribonuclease, Plasmid
Document Summary
Classic phenotypes are color, shape, morphology, and growth. Genes are always written lower case and italicized. Wild type is assumed unless something is said about it. Even wild types can have no expression in terms of phenotype. Z followed by a number shows the mutation in that gene aka that allele. Ynbn is the gene from her phd and gives resistance that we will use in lab. Lac+ means that the bacterium can utilize lactose but is not necessarily having the gene. Carbon source for macconkey is lactose and peptone: peptone used creates ammonia why has a higher ph lacz (gene) lacz (protein) nomenclature. Plasmid can replicate on its own even though it may use host enzymes. No one looked into microbial genetics because they thought prokaryotes are simple and just replicate blah blah. Avery: did a simple transformation test and the cells that had no transformation only occurred after a dnase was added indicating that the.
