GEN-3020 Lecture 12: CHP 11 (DNA Replication)
11 Oct 2016
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Document Summary
In e. coli, dna is replication at a rate of 1000 nucleotides per second. Arrangement and nature of nitrogenous bases allow. Complementarity of dna strands allows each strand to serve as template for synthesis of the other. Semiconservative: each replicated dna molecule consists of o(cid:374)e (cid:862)old(cid:863) a(cid:374)d o(cid:374)e (cid:374)e(cid:449) st(cid:396)a(cid:374)d. Conservative: two newly synthesized strands come together original helix is conserved. Dispersive: parental strands are dispersed into two new double helices. Meselson and stahl (1958: 15n-labeled e. coli grown in medium containing 14n, each new dna molecule consists of one old and one newly synthesized strand, provided strong evidence that dna is semiconservative in prokaryotes. Taylor-woods-hughes (1957: vicia faba (broad bean) was used to demonstrate dna replication is semiconservative in eukaryotes, monitored process of replication with labeled. Dna replication begins at the origin of replication. Polymerases i, ii, iv, and v are involved in various aspects of dna repair (from external forces like uv light).
