GEN-3000 Lecture Notes - Lecture 4: Pyrimidine Dimer, Ethidium Bromide, Kozak Consensus Sequence
20 Oct 2017
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Initiation of translation- components assemble - mrna, small and large subunits of the ribosomes, initiation factors, initiator trna and gtp: 3 major steps, mrna binds to small ribosomal subunit, large ribosome joins initiator complex. For next step, initiation factor 2 (if-2) complexes with gtp: initiator fmet-trnafmet attaches to initiation codon, all of this (+mrna, small ribosome) = 30s initiation complex xi. 70s initiation complex - large subunit associates after if-3 dissociates xii. 30s initiation sequence small subunit binds with initiation factors and mrna to position the large subunit: fmet=charged trna xiii. Infectious - acquired by eating/transfusion/growth hormone: heritable, fatal familial insomnia insomnia caused by misfolded proteins, lose ability to sleep at all, death occurs within 7-36 months; prevents rem sleep, ch. Introduce damage to dna by natural or artificially induced oxidative radicals. If (cid:272)o(cid:373)pou(cid:374)d (cid:272)auses ge(cid:374)e (cid:272)ha(cid:374)ges, the(cid:396)e (cid:449)ill (cid:271)e (cid:396)e(cid:448)e(cid:396)ta(cid:374)ts that (cid:862)fi(cid:454)ed(cid:863) the p(cid:396)o(cid:271)le(cid:373)s i(cid:374) the bacteria: transposable elements- repetitive and possibly mobile dnas, present in all organisms (ex.
