GEN-3000 Lecture Notes - Lecture 10: Nucleosome, Exonuclease, Dna Mismatch Repair
15 Mar 2017
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Document Summary
Bacteria replicated 1000 nucleotides/second . 1 in 1 billion error! Meselson and stahl could rule out conservative replication, bc both new strands. Taylor-woods-hughes showed semiconservative replication in eukaryotes and neither of them was same as original dna. Each chromatin has one strand labeled - label on both sides. Reminds us that you always have to have an origin of replication. One origin opens up = by direction (expands in both directions), but it only has one origin so the whole organism must be replicated at one time. Used by some viruses, and f factor. Replication has many requirements: ssdna template - single stranded dna template, dntps - need all four bases (a,c,t,g), need them to be triphosphates (for atp, gtp, enzymes - machinery . Synthesis of dna goes in opposite directions (bidirectional) We can only make dna 5" 3". Okazaki fragments pieces of dna on lagging strand.
