GEN-3000 Lecture Notes - Lecture 14: Replica Plating, Antimicrobial Resistance, Plasmid
Document Summary
Aabbcc can also be indicated as abc/abc. Linkage mapping: 1 % recombination = 1 centimorgan, the female map is 1. 5 times the size of the male map in drosophila. 1 cm = 1 million base pairs: recombination hot spots are areas where recombination occurs more often than expected. This is due to negative interference: in humans, we tend to see more positive interference than negative interference. Positive interference leads to less recombination than expected. Collinear maps: more compact in the male. Both linkage maps and collinear maps show the same genes in the same order. Genetic map of drosophila chromosome: we are not limited to mapping physical traits, we can use molecular markers, which are still based on the segregation of two or more markers. We have to use molecular markers to detect alleles in different individuals and to go backwards to create pedigrees in humans: rflp changes in the dna sequence that modify restriction enzyme sites.
