BCHM-3050 Lecture Notes - Lecture 2: Dna Polymerase Iii Holoenzyme, Dna Polymerase Ii, Glycosidic Bond
20 Oct 2017
School
Department
Course
Professor
Document Summary
Last (cid:374)u(cid:272)leotide (cid:449)ill al(cid:449)a(cid:455)s ha(cid:448)e a f(cid:396)ee (cid:1007)" oh g(cid:396)oup (cid:374)ot i(cid:374)(cid:448)ol(cid:448)ed i(cid:374) phosphodieste(cid:396) (cid:271)o(cid:374)d: b-dna dimensions most native dna in the cell. Rna"s (cid:894)(cid:373)essages, t(cid:396)a(cid:374)sfe(cid:396) species, ribosome numbers) made than the 2 copies of dna: modified bases in trna include pseudouridine, dihydrouridine, 4-thiouridine, 1&2-methylguanosines especially in loops: u is replaced by t (i. e. methylated uracil), because t will only base-pair with a; U, however is the only base that can base pair with any of the other bases. E(cid:448)olutio(cid:374)a(cid:396)il(cid:455), this (cid:272)ould (cid:374)ot (cid:271)e (cid:862)tole(cid:396)ated(cid:863) to (cid:373)ai(cid:374)tai(cid:374) the fidelit(cid:455) (cid:396)e(cid:395)ui(cid:396)ed i(cid:374) Dna base pairing: in a single point in time there is 5x more rna than dna in the cell ix. Rna needs to be able to move out of nucleus, using one dna template, many. Triple stranded dna a (cid:862)triple helix(cid:863: discovered in 1957 (shortly after watson & Crick): thought to form from partially unwound duplex.
