CAS BI 203 Lecture Notes - Lecture 1: Rous Sarcoma Virus, Sidney Altman, Thomas Cech
Document Summary
~ molecular medicine and lab experiments from textbook ~ Lecture 1: the cell: viruses: intracellular parasites that cannot replicate alone. They reproduce by infecting host cells and using the cellular machinery of host to replicate. Most carry only three or four genes including proteins, only thousands of base pairs: bacteriophage: viruses that can infect bacteria, retroviruses: rna genomes but synthesize dna copy of their genome in infected cells. Ultimately lead to disease: rous sarcoma virus: connective tissue cancer, quite rare. Simplest model to study dna replication is bacteria: prokaryotic cells: lack nuclear envelope, small, no cytoplasmic organelles, single circular. Will be able to see those cells on a plate (primary culture). If these cells divide we can use this to create a secondary culture. No relation between number of protein coding genes and complexity of. Organism: frap (fluorescence recovery after photobleaching, you can bleach portions of the cell, which stops light from being emitted.
