BIOL 401 Lecture Notes - Lecture 12: Fluorophore, Acrylamide, Dna Microarray
16 Mar 2017
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We can use data integration to find biomarkers for better diagnostics, better understand disease mechanisms, and find new therapeutics. Southern blots: use dna to probe a dna blot. Can use looking at cross species (ex. clown beetles). Take the sequence of one clown beetle and make it the probe for the other beetle"s sequence. Northern blot: use dna probe to examine gene expression. Probe rna gels for transcripts. qpcr can be used. Use a dna probe to probe an rna blot. Rna is produced in a tissue at any given time. Ex. gene x is differentially regulated between a control and patient with. A larger, darker band suggests there is more rna in the. Western blots: uses antibodies to localize production of a specific protein. Ex. see there is a large band in alzheimer"s disease tissue from the previous experiment.
